Sperm-binding to the perivitelline membrane of chicken egg yolk as a functional test for sperm evaluation in dogs
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چکیده
منابع مشابه
Freezing Bovine Sperm with Egg Yolk
Bull spermatozoa were pellet frozen on dry ice in a TES buffer containing combinations of egg yolk and glycerol to determine which component of an extender afforded cryoprotection to the spermatozoa. Neither buffer nor buffer plus glycerol protected sperm cell motility in the absence of egg yolk, while sperm cells frozen in egg yolk buffer in the absence of glycerol had 24% postthaw motility (P...
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15 صفحه اولIdentification of perivitelline N-linked glycans as mediators of sperm-egg interaction in chickens.
This study demonstrates that carbohydrates play an essential role in sperm-egg interactions in birds. Sperm-egg interaction was measured in vitro as the ability of spermatozoa to hydrolyse a small hole in the inner perivitelline layer, the equivalent of the mammalian zona pellucida. Preincubation with Triticum vulgaris lectin (WGA) and succinyl-WGA (S-WGA) at 10 microgram ml(-1) resulted in com...
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Past studies have suggested that mouse sperm surface galactosyltransferase may participate during fertilization by binding N-acetylglucosamine (GIcNAc) residues in the zona pellucida. In this paper, we examined further the role of sperm surface galactosyltransferase in mouse fertilization. Two reagents that specifically perturb sperm surface galactosyltransferase activity both inhibit sperm-zon...
متن کاملA role for mouse sperm surface galactosyltransferase in sperm binding to the egg zona pellucida
Past studies have suggested that mouse sperm surface galactosyltransferase may participate during fertilization by binding N-acetylglucosamine (GlcNAc) residues in the zona pellucida. In this paper, we examined further the role of sperm surface galactosyltransferase in mouse fertilization. Two reagents that specifically perturb sperm surface galactosyltransferase activity both inhibit sperm-zon...
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ژورنال
عنوان ژورنال: Brazilian Journal of Veterinary Research and Animal Science
سال: 2017
ISSN: 1678-4456,1413-9596
DOI: 10.11606/issn.1678-4456.bjvras.2017.127331